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American Journal of Public Health, Vol. 72, Issue 12 1406-1407, Copyright © 1982 by American Public Health Association

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Simplified method for Chlamydia trachomatis isolation using multi-well plate.

H Hayashi and P K Sheth

To simplify Chlamydia trachomatis isolation, we employed a 24-well tissue culture plate, thereby eliminating tedious steps and material in the conventional vial method. Parallel studies indicated that the vial and plate methods were equally sensitive for Chlamydia isolation. The time for culturing McCoy cells was reduced from 72 to four hours allowing us to inoculate specimens the same day. The potential hazard of handling coverslips which might contain infected cells was avoided.






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Copyright © 1982 by the American Public Health Association